When it comes to wrinkles, a lot of it is pure genetics. Have you heard the saying, "you either save your face or your fanny"?
As for Botox alternatives, I really love dee2do's suggestions!! Anything that leaves you calmer and reduces stress has to be a good approach. Stress can really do a number on your health - and your skin.
I'd also add in there: diet. A diet rich in fruits & veggies and omega 3 fats can help reduce stress and wrinkles, from the inside out.
Otherwise, I can say that as you get older it's a really good idea to change your skin care routine to something deeper, richer and more moisturizing. What worked in your 20s is not ideal for your 30s and 40s, and I'm sure what works in your 40s probably isn't ideal for your 50s and 60s either.
Once I hit my 40s, I found I needed a much richer moisturizer. I also started exfoliating around then - which does make a big difference, maybe not so much with fine lines and wrinkles but with all over appearance and your "glow."
For laugh lines, I'm lately experimenting with serums, though I am not sure I have the hang of it quite yet - I've been getting break-outs around my chin since I started experimenting, so I'm thinking the serums are best in small doses in small areas, like eye area or 11 area only.
You don't necessarily need expensive department brand stuff, but I did splurge on Chanel Hydromax moisturizer this summer when in Paris, and have grown to really love it, and just bought a second bottle. It is rich and ultra-moisturizing, but doesn't clog pores or irritate sensitive skin.
On the drug-store end of the spectrum, I would also highly recommend trying any of the Oil of Olay product lines. I have been using OoO daily moisturizer with SPF since my 20s, and that is the brand exfoliator I use too. Also, they have a 12-day program (I think it's 12 days? Don't know the name, but it comes in separate, tiny vials). According to my completely unbiased, objective DH, it did miracles (ahem), so is definetly worth a try. (Okay so he is NOT unbiased but, I don't think he was merely flattering me, since now he wants to try it himself too).
If nothing else, all the self-pampering of trial and error will reduce stress and relax you!!